Characterization of Growth-Promoting Activities of Consortia of Chlorpyrifos Mineralizing Endophytic Bacteria Naturally Harboring in Rice Plants—A Potential Bio-Stimulant to Develop a Safe and Sustainable Agriculture

Eighteen pesticide-degrading endophytic bacteria were isolated from the roots, stems, and leaves of healthy rice plants identified through 16S rRNA gene sequencing. Furthermore, biochemical properties, including enzyme production, dye degradation, anti-bacterial activities, plant-growth-promoting traits, N-fixation, P-solubilization, auxin ACC-deaminase activities these naturally occurring along with their four consortia, characterized. Enterobacter cloacae HSTU-ABk39 sp. HSTU-ABk36 displayed inhibition zones 41.5 ± 1.5 mm, 29 09 mm against multidrug-resistant human pathogenic Staphylococcus aureus epidermidis, respectively. FT-IR analysis revealed that all eighteen isolates able to degrade chlorpyrifos pesticide. Our study confirms play a key role in enhancing plant growth. Notably, grown pots containing reduced urea (30%) mixed either bacterial consortium-1, consortium-2, consortium-3, or consortia-4 demonstrated an increase 17.3%, 38.6%, 18.2%, 39.1% yields, respectively, compared control 100% fertilizer. GC–MS/MS confirmed consortia treatment caused degradation into different non-toxic metabolites, 2-Hydroxy-3,5,6 trichloropyridine, Diethyl methane phosphonate, Phorate sulfoxide, Carbonochloridic. Thus, could be deployed as bio-stimulants improve crop production by creating sustainable biological system.